A novel chromatography system to isolate active ribosomes from pathogenic bacteria
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چکیده
منابع مشابه
A novel chromatography system to isolate active ribosomes from pathogenic bacteria.
We have developed a novel chromatography for the rapid isolation of active ribosomes from bacteria without the use of harsh conditions or lengthy procedures that damage ribosomes. Ribosomes interact with an alkyl linker attached to the resin, apparently through their RNA component. Examples are given with ribosomes from Escherichia coli, Deinococcus radiodurans, and with clinical isolates of St...
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16 In a current experiment, we have found that a protein fraction isolated from the 105,000 X g supernatant can catalyze a similar RNA synthesis in the presence of four nucleoside triphosphates and the E. coli DNA prepared by the method of Marmur (J. Mol. Biol., 3, 208, 1961). In this system also, the addition of salmine enhances the initial rate of the synthesis and prevents the degradation of...
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ژورنال
عنوان ژورنال: RNA
سال: 2007
ISSN: 1355-8382
DOI: 10.1261/rna.692408